The Immortalized Porcine Skeletal Muscle Satellite Cells, cataloged as CSC-I2067Z, represent a significant advancement in muscle regeneration research. Derived from porcine skeletal muscle, these cells maintain their functional capabilities long-term, making them an essential resource for scientists studying muscle development and repair.
Key Features
Origin and Immortalization
These cells are isolated from the longissimus dorsi muscle of one-day-old Western commercial pigs using the differential adhesion method. The immortalization process is achieved through stable transduction with SV40 T-antigen using lentiviral vectors. This technique allows the cells to proliferate beyond the limitations typically seen in primary cultures.
Morphology and Culture
Characteristically, these cells exhibit polygonal morphology and adhere well in culture. They are cultivated in a specialized medium, the SuperCult™ Immortalized Porcine Skeletal Muscle Cell Medium (Cat No.: CM-I2067Z). Their culture properties enable efficient handling and reproduction across multiple passages.
Research Applications
The operational capacity of these immortalized cells offers a myriad of applications in biomedical research, including:
- Muscle Regeneration Studies: These cells are pivotal in analyzing mechanisms behind muscle growth and repair.
- Cultured Meat Production: Their ability to proliferate indefinitely opens doors for sustainable meat production practices.
- Disease Modeling: The cells can serve as a model for studying muscular diseases specific to porcine species.
- Drug Testing: Utilized in pharmacological studies to assess drug effects on muscle cells.
Scientific Verification
Research conducted on the SV40 T-pMuSCs (immortalized porcine skeletal muscle satellite cells) indicates that they can be sub-cultured over forty generations without significant loss of proliferative capabilities. Immunofluorescence assays and quantitative PCR analyses confirm their physiological relevance comparable to primary muscle satellite cells.
Quality Control and Customer Support
The manufacturing process emphasizes stringent quality control, ensuring that every batch meets high standards for morphology, growth rate, and genetic stability. Creative Bioarray provides comprehensive support, assisting customers with experimental design and troubleshooting.
Conclusion
The Immortalized Porcine Skeletal Muscle Satellite Cells (CSC-I2067Z) represent an invaluable tool for researchers. Their ability to reliably replicate under controlled conditions aligns with the needs of contemporary biomedical research, making these cells essential for advancing our understanding of muscle biology and therapeutic interventions.